نقد و بررسی اجمالی
Laboratory Exercises in Microbiology – 10th Edition
by John Harley (Author)
Laboratory Exercises in Microbiology – 10th Edition was designed and written to be directly correlated to Prescott’s Microbiology, tenth Edition, by Joanne M. Willey, Linda M. Sherwood, and Christopher J. Woolverton. The class-tested exercises are modular to allow instructors to easily incorporate them into their course. This balanced introduction to each area of microbiology now also has accompanying Connect content for additional homework and assessment opportunities.
About the Author
John Harley teaches Anatomy & Physiology and General Biology at Eastern Kentucky University, Richmond, KY and is also co-author of Prescott / Harley / Klein Microbiology and the ASM Microbial Telecourse Study Guide.
PART ONE Microscopic Techniques
۱. Bright-Field Light Microscope (Dark Image, Brighter Background) and Microscopic Measurement of Organisms
۲. The Hanging Drop Slide and Bacterial Motility
۳. Dark-Field Light Microscope: Bright Image, Dark Background
۴. Phase-Contrast Light Microscope
۵. Fluorescence Microscope
PART TWO Bacterial Morphology and Staining
۶. Negative Staining
۷. Smear Preparation and Simple Staining
۸. Gram Stain
۹. Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
۱۰. Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
۱۱. Capsule Staining
۱۲. Flagella Staining: West and Difco’s SpotTest Methods
PART THREE Basic Laboratory and Culture Techniques
۱۳. Sterilization and Microbiological Culture Media Preparation
۱۴. Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
۱۵. Spread-Plate Technique
۱۶. The Streak-Plate Technique: Selective and Differential Media
۱۷. Pour-Plate Technique
۱۸. Cultivation of Anaerobic Bacteria
۱۹. Determination of Bacterial Numbers
PART FOUR Biochemical Activities of Bacteria
۲۰. Carbohydrates I: Fermentation and β-Galactosidase Activity
۲۱. Carbohydrates II: Triple Sugar Iron Agar Test
۲۲. Carbohydrates III: Starch Hydrolysis
۲۳. Lipids: Lipid Hydrolysis
۲۴. Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
۲۵. Proteins, Amino Acids, and Enzymes II: The IMViC Tests
۲۶. Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
۲۷. Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
۲۸. Proteins, Amino Acids, and Enzymes V: Catalase Activity
۲۹. Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
۳۰. Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence
۳۱. Proteins, Amino Acids, and Enzymes VIII: Urease Activity
۳۲. Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests
۳۳. Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
۳۴. Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
PART FIVE Rapid Multitest Systems
۳۵. The API® 20E System
۳۶. The EnteroPluri-Test System
PART SIX Unknown Identification
۳۷. Using the First Edition of Bergey’s Manual of Systematic Bacteriology to Identify Bacteria
۳۸. General Unknown
PART SEVEN Environmental Factors Affecting Growth of Microorganisms
۳۹. Temperature
۴۰. pH
۴۱. Osmotic Pressure
۴۲. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products
۴۳. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) and the Etest®
۴۴. Hand Washing, Environmental Sampling, and Microbiological Monitoring
۴۵. Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
PART EIGHT Environmental and Food Microbiology
۴۶. Standard Coliform Most Probable Number (MPN) Test and Colilert®-۱۸ Coliform Test
۴۷. Membrane Filter Technique for Coliforms and Fecal Streptococci; KONFIRMTM Test for Fecal Coliforms
۴۸. Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
۴۹. Enumeration of Soil Microorganisms
۵۰. Bacterial Count of a Food Product
۵۱. Examination of Milk for Bacteria
PART NINE Medical Microbiology
۵۲. Hemagglutination Reactions: Blood Groups
۵۳. Isolation of Normal Microbiota from the Human Body
۵۴. Staphylococci
۵۵. Pneumococci
۵۶. Streptococci
۵۷. Neisseriae
۵۸. Aerobic and Anaerobic Endospore-Forming Bacteria
PART TEN Survey of Selected Eucaryotic Microorganisms
۵۹. Fungi (Ascomycota; also Ascomycetes) I: Yeasts
۶۰. Fungi (Eumycota) II: Zygomycota (Rhizopus), Ascomycota (Penicillium), and Basidiomycota(Agaricus)
PART ELEVEN Microbial Genetics and Genomics
۶۱. Bacterial Mutation
۶۲. Bacterial Transformation
۶۳. Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
۶۴. Isolation of Genomic DNA from Baker’s Yeast (Saccharomyces cerevisiae)
۶۵. Isolation and Purifi cation of Genomic DNA from Escherichia coli
PART TWELVE Scientific Inquiry
۶۶. Independent Group Projects
۶۷. Case Studies
Appendix A Dilutions with Sample Problems
Appendix B Transmission-Absorbance Table for Spectrophotometry
Appendix C Logarithms; pH and pH Indicators
Appendix D Scientifi c Notation
Appendix E Identifi cation Charts
Appendix F Reagents, Solutions, Stains, and Tests
Appendix G Culture Media Appendix H Sources and Maintenance of Microbiological Stock Cultures
Appendix I Laboratory Skills and Laboratory Thinking Skills
Appendix J Orientation to the Laboratory: Rules of Conduct and General Safety
Appendix K Summary of Universal Precautions and Laboratory Safety Procedures
Appendix L Biosafety Level (BSL)
Index
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